The Effect of Human Salivary Exosome on The Basic Fibroblast Growth Factor and Collagen Type I Gene Expressions from Human Periodontal Fibroblast Cells

Abstract

Gene expression is the most fundamental level at which the genotype gives rise to phenotype. The development of human salivary exosomes has become one of the promising researches to improve cell-based tissue engineering but their functions in human periodontal ligament fibroblast (HPdLF) cells are not well studied. To study the effect of human salivary derived exosomes on the gene expression of HPdLF cells. In vitro, HPdLF cells were cultured for 24 hours with 10 µg/ml of human salivary exosomes. Determination of gene expression levels of basic fibroblast growth factor (bFGF) and collagen type I (COL1) in the presence and absence of human salivary exosomes in HPdLF culture was performed using quantitative reverse transcriptase polymerase chain reaction (RT-qPCR). Human salivary exosomes significantly upregulated bFGF gene expression but not COL1 gene in HPdLF cells after 24 hours of culture. Human salivary exosomes are able to upregulate bFGF gene in HPdLF cells. Thus, they might have potential to be used as an alternative biomaterial in tissue engineering for periodontal regeneration.