Prevalence of Reduced Vancomycin Susceptibility measured by minimum inhibition concentration (MIC) among Methicillin Resistant Staphylococcus aureus (MRSA) isolates from Surgical Site Infected Patients at Clinical Training Centre, Sungai Buloh, Malaysia

Siti Farah Alwani Mohd Nawi, Nur Hazirah Kamal Bahrin, Farah Amirah Ahmad

Abstract


Methicillin Resistant Staphylococcus aureus (MRSA) causing surgical site infections is one of the most common nosocomial infections affecting post-surgery patients. Vancomycin is the recommended treatment with MRSA-resistance breakpoint for minimum inhibition concentration (MIC) of 2 ug/mL where the pathogen can be considered as susceptible. Here, we describe the MIC of vancomycin against our MRSA isolates. Retrospective data of MRSA positive cultures from post-surgical patients who were admitted to the Clinical Training Centre Sungai Buloh public section from 2016-2017 with documented MIC to vancomycin were analyzed. The specimens consist of pus swabs, mediastinal fluid, sternal bone, and tissue. A total of 29 MRSA were isolated from 11 patients. There were 19, 3, 3 and 4 MRSA with vancomycin’s MIC (ug/mL) of 0.5, 1, 1.5 and 2 respectively. The MRSA with MIC of 2 ug/mL were observed from two different patients with one of them showing MIC of 0.5 ug/mL which grew from wound swabs to 2.0 ug/mL which grew from sternal bone and mediastinal fluid isolates. Vancomycin reduced susceptibility MRSA has been observed in our clinical training centre with a 1.1% incidence. Identification of possible risk factors and follow up of outcomes is required to fully elucidate the importance of this occurrence.

Keywords


Methicillin Resistant Staphylococcus aureus (MRSA), Reduced vancomycin susceptibility, Minimum inhibition Concentration (MIC), Surgical site infections (SSI)

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