Biocompatibility Assessment of Newly Fabricated Melt Derived 45S5 Bioactive Glass on Dental Cells Proliferation

Nurul Shazwani Mohd Zain, Siti Syazni Tajudin, Siti Noor Fazliah Mohd Noor, Hasmaliza Mohamad

Abstract


Abstract—The purpose of this study was to fabricate bioactive glass and to assess the bioactive glass (BG) biocompatibility through the assessment of proliferation rate of stem cells from human exfoliated deciduous teeth (SHED) in the presence of five different concentrations of 45S5 bioactive glass conditioned medium (1 to 10 mg/ml powder to liquid ratio) using alamarBlue assay. 45S5 bioactive glass known as Bioglass® in mole percentages of 46.13% SiO2, 26.91% CaO, 24.35% Na2O and 2.60% P2O5 was fabricated using melt-quenching technique and then the glass was characterized by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) confirming the composition and its amorphous state. Stem cells from human exfoliated deciduous tooth (SHED) were used to evaluate the biocompatibility of 45S5 by exposing the cells to various concentration of BG-conditioned medium (1-10 mg/ml) using alamarBlue assay. XRD result indicated that the BG produced has an amorphous structure. FTIR analysis showed the Si-O-Si bending, Si-O stretching and Si-O-Si stretching (asymmetric) bands present within the BG structure indicating the characteristic of silicate network. For alamarBlue assay, SHED exposed to BG-conditioned medium showed increasing proliferative activity at lower concentration of BG powder to liquid ratio from Days 1 to 7. Hence, it can be concluded that the 45S5 BG with lower concentration of powder to liquid ratio promoted SHED proliferation which can be used for future work.

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References


Yamada, Y., Ito, K., Nakamura, S., Ueda, M. and Nagasaka T. Promising cell-based therapy for bone regeneration using stem cells from deciduous teeth, dental pulp, and bone marrow. Cell transplantation. 2011; 20(7):pp.1003-1013.

Rahaman, M.N., Day, D.E., Bal, B.S., Fu, Q., Jung, S.B., Bonewald, L.F. and Tomsia, A.P. Bioactive glass in tissue engineering. Acta biomaterialia. 2011; 7(6): pp.2355-2373.

Fu, Q., Saiz, E., Rahaman, M.N. and Tomsia, A.P. Bioactive glass scaffolds for bone tissue engineering: state of the art and future perspectives. Materials Science and Engineering. 2011; C, 31(7): pp.1245-1256.

Pirayesh, H. and Nychka, J.A. Sol–Gel Synthesis of Bioactive Glass‐Ceramic 45S5 and its in vitro Dissolution and Mineralization Behavior. Journal of the American Ceramic Society. 2013; 96(5): pp.1643-1650.

Cao, W. and Hench, L.L. Bioactive materials. Ceramics international. 1996; 22(6): pp.493-507.

Jones, J.R., Lee, P.D. and Hench, L.L. Hierarchical porous materials for tissue engineering. Philosophical Transactions of the Royal Society of London A: Mathematical, Physical and Engineering Sciences. 2006; 364(1838): pp.263-281.

Wu, Z.Y., Hill, R.G. & Jones, J.R. Optimizing the Processing of Porous Melt-Derived Bioactive Glass Scaffolds. Bioceramics Development and Applications. 2010; 1: pp.1–4.

Hench and L.L.. The story of Bioglass®. Journal of Materials Science: Materials in Medicine. 2006; 17(11): pp.967-978.

Xynos, I.D., Hukkanen, M.V.J., Batten, J.J., Buttery, L.D., Hench, L.L. and Polak, and J.M. Bioglass® 45S5 stimulates osteoblast turnover and enhances bone formation in vitro: implications and applications for bone tissue engineering. Calcified Tissue International. 2000; 67(4): pp.321-329.

Peitl Filho, O., Latorre, G.P. and Hench, and L.L.. Effect of crystallization on apatite-layer formation of bioactive glass 45%. J Biomed Mater Res. 1996; 30:pp.509-514.

Bingel, L., Groh, D., Karpukhina, N. and Brauer, D.S. Influence of dissolution medium pH on ion release and apatite formation of Bioglass® 45S5. Materials Letters. 2015; 143: pp.279-282.

Bahniuk, M.S., Pirayesh, H., Singh, H.D., Nychka, J.A. and Unsworth, L.D. Bioactive glass 45S5 powders: effect of synthesis route and resultant surface chemistry and crystallinity on protein adsorption from human plasma. Biointerphases. 2012; 7(1): p.41.

Nakamura, S., Yamada, Y., Katagiri, W., Sugito, T., Ito, K. and Ueda, M. Stem cell proliferation pathways comparison between human exfoliated deciduous teeth and dental pulp stem cells by gene expression profile from promising dental pulp. Journal of endodontics. 2009; 35(11): pp.1536-1542.

Miura, M., Gronthos, S., Zhao, M., Lu, B., Fisher, L.W., Robey, P.G. and Shi, S., 2003. SHED: stem cells from human exfoliated deciduous teeth. Proceedings of the National Academy of Sciences. 2003; 100(10): pp.5807-5812.

Schickle, K., Zurlinden, K., Bergmann, C., Lindner, M., Kirsten, A., Laub, M., Telle, R., Jennissen, H. and Fischer, H. Synthesis of novel tricalcium phosphate-bioactive glass composite and functionalization with rhBMP-2. Journal of Materials Science: Materials in Medicine. 2011; 22(4): pp.763-771.

Fan, J.P., Kalia, P., Di Silvio, L. and Huang, J. In vitro response of human osteoblasts to multi-step sol–gel derived bioactive glass nanoparticles for bone tissue engineering. Materials Science and Engineering: C. 2014; 36: pp.206-214.

El-Kady, A.M., Ali, A.F., Rizk, R.A. and Ahmed, M.M. Synthesis, characterization and microbiological response of silver doped bioactive glass nanoparticles. Ceramics International. 2012; 38(1): pp.177-188.

Srivastava, A.K. and Pyare, R., 2012. Characterization of ZnO Substituted 45S5 Bioactive glasses and glass-ceramics. Journal of Materials Science Research. 2012; 1(2): p.207.

Casagrande, L., Cordeiro, M.M., Nör, S.A. and Nör, J.E. Dental pulp stem cells in regenerative dentistry. Odontology. 2011; 99(1): pp.1-7.

Boccaccini, A.R., Erol, M., Stark, W.J., Mohn, D., Hong, Z. and Mano, J.F. Polymer/bioactive glass nanocomposites for biomedical applications: a review. Composites science and technology




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