Expression of odontogenic markers in stem cells from human exfoliated deciduous teeth treated with perivitelline fluid from horseshoe crab

Ibrahim N, Mohamad Nasir NF, Azmi NI, Kannan TP

Abstract


Stem cells from human exfoliated deciduous teeth (SHED) makes an ideal source for mesenchymal stem cells (MSCs) for cell-based regenerative therapies due to their ability to differentiate into wide range of cells lineages. Teeth regeneration requires regulated odontogenesis that will generate dentin. Previous studies have shown the potential of perivitelline fluid (PVF) to act as a supplement material in enhancing proliferation of cells and promoting the generation of certain organs due to its high primitive protein content. This study analysed the expression of odontogenesis markers in SHED treated with PVF (0.019 mg/mL) on day 1, 3, and 7. The expression of dentin sialophosphoprotein (DSPP), dentin matrix acidic phosphoprotein 1 (DMP-1), osteopontin (OPN) and runt-related transcription factor 2 (RUNX2) were analysed at the transcript level in two groups of SHED which are PVF treated group (0.019 mg/ml) and without PVF. All four genes expressed at higher level in treatment group as compared to control on all tested days. Significant difference in gene expression between the control and treated groups were found on all tested days except for RUNX2 gene whereby its expression was not significantly different at day 7. Thus, it is suggested that PVF has the potential to enhance odontogenesis in SHED.

Keywords


Deciduous teeth, gene expression, horseshoe crab, odontogenesis, perivitelline fluid, stem cells

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References


Huang GJ, Gronthos S, Shi S. Mesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medicine. J Dent Res. 2009; 88: pp.792-806.

Miura M, Gronthos S, Zhao M, Lu B, Fisher LW et al. SHED: stem cells from human exfoliated deciduous teeth. Proc Natl Acad Sci. 2003; 100: pp.5807-5812.

Semeghini MS, Fernandes RR, Chimello DT, Oliveira F Sd, Bombonato-Prado KF. In vitro evaluation of the odontogenic potential of mouse undifferentiated pulp cells. Braz Dent J. 2012; 23: pp.328-336.

Musa M, Ali KM, Kannan TP, Azlina A, Omar NS et al. Effects of perivitelline fluid obtained from horseshoe crab on the proliferation and genotoxicity of dental pulp stem cells. Cell J (Yakhteh). 2015; 17: pp.253-263.

Ghaskadbi S, Patwardhan V, Chakraborthy M, Agrawal S, Verma MK et al. Enhancement of vertebrate cardiogenesis by a lectin from perivitelline fluid of horseshoe crab embryo. Cell Mol Life Sci. 2008; 65: pp.3312-3324.

Srijaya T, Pradeep P, Hassan A, Chatterji A, Shaharom F. Effect of peri-vitelline fluid from horseshoe crab embryo in enhancing the early gonadal development in red tilapia. Biol Forum Int J. 2013; 5: pp.1-7.

Nagai T, Kawabata S, Shishikura F, Sugita H. Purification, characterization, and amino acid sequence of an embryonic lectin in perivitelline fluid of the horseshoe crab. J Biol Chem.1999; 274: pp.37673-37678.

Chatterji A, Vijayakumar R, Parulekar A. Growth and morphometric characteristic in the horse-shoe crab, Carcinoscorpius rotundicanda (Latreille) from Canning (West Bengal), India. Pak J Sci Ind Res. 1988; 31: pp.352-353.

Guo L, Li J, Qiao X, Yu M, Tang W et al. Comparison of odontogenic differentiation of human dental follicle cells and human dental papilla cells. PLoS One. 2013; 8: pp.e62332.

Zainal Ariffin SH, Kermani S, Zainol Abidin IZ, Megat Abdul Wahab R, Yamamoto Z et al. Differentiation of dental pulp stem cells into neuron-like cells in serum-free medium. Stem Cells Int. 2013; 2013: pp.1-10.

Lee SK, Lee KE, Song SJ, Hyun HK, Lee SH et al. A DSPP mutation causing dentinogenesis imperfecta and characterization of the mutational effect. BioMed Res Int. 2012; 2013: pp.1-7.

Inagaki Y, Kashima T, Hookway E, Tanaka Y, Hassan A et al. Dentine matrix protein 1 (DMP 1) is a marker of bone formation and mineralisation in soft tissue tumours. Virchows Arch. 2015; 466: pp.445-452.

Chen S, Gluhak-Heinrich J, Wang Y, Wu Y, Chuang H et al. Runx2, osx, and dspp in tooth development. J Dent Res. 2009; 88: 904-909.

Reinholt FP, Hultenby K, Oldberg A, Heinegård D. Osteopontin--a possible anchor of osteoclasts to bone. Proc Natl Acad Sci. 1990; 87: pp.4473-4475.

Bahrambeigi V, Salehi R, Hashemibeni B, Esfandiari E. Transcriptomic comparison of osteopontin, osteocalcin and core binding factor 1 genes between human adipose derived differentiated osteoblasts and native osteoblasts. Adv Biomed Res. 2012; 1: pp.8.

Lu Y, Zhang S, Xie Y, Pi Y, Feng JQ. Differential regulation of dentin matrix protein 1 expression during odontogenesis. Cells Tissues Organs. 2005; 181: pp.241-247.




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Journal of Biomedical and Clinical Sciences (JBCS) publishes original research articles, short review, short communication, case reports, quiz and letter to editor that covering the areas of fundamental aspects of biomedical and clinical sciences, from pre-clinical towards translational research and their applications in medicine, dentistry and healthcare management. JBCS aims to provide a platform for basic science and clinical researchers to communicate and share their research findings to the advancement of technologies in biomedical and clinical sciences by providing maximum access to scholarly communication.

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