Antibacterial activity of heterogeneous TiO2 and ZnO nanoparticles against Gram-positive and Gram-negative bacterial pathogens

Nor Hazliana Harun, Rabiatul Basria S.M.N. Mydin, Srimala Sreekantan, Khairul Arifah Saharudin, Yong Ling Khor, Norfatehah Basiron, Azman Seeni

Abstract


Hospital-acquired infections (HAIs) are responsible for over 40% of cases in acute-care hospitals and commonly associated with catheters-associated urinary tract infections (CAUTIs). Current nanotechnology approach focus on improving the aseptic procedures for medical devices and manage the HAIs risk. TiO2 and ZnO nanoparticles (NPs) have been widely reported independently, to have a photocatalytic killing potential. The present study evaluates the antibacterial activity of heterojunction between TiO2 and ZnO NPs on several types bacterial pathogens model including Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. The antibacterial screening test on TiO2/ZnO nanoparticles (NPs) were done under dark and light conditions with different molar ratio 25T75Z, 50T50Z and 75T25Z according to Clinical Laboratory Standards Institute (CLSI) guidelines MO2-A11. ZnO and TiO2/ZnO (25T75Z and 50T50Z) NPs at the highest concentration (1000μg/μL) showed mean diameters of the zones of inhibition (mm); (12.5 ± 0.58), (12.13 ± 0.85), and (7.25 ± 1.44) in dark condition. Increment in inhibition zones was obtained under light condition; (21.38 ± 0.48), (17.50 ± 1.0), and (12.38 ± 1.80). Findings from this study highlights the heterogeneous TiO2 and ZnO NPs could become a promising bacteriostatic and/or bactericidal agent to combat against the HAIs.

Keywords


TiO2/ZnO nanoparticles; Hospital-acquired infections; Antibacterial activity; Bacteriostatic agent; Bactericidal agent; Biomedical Product; Biomaterial; Nanomaterial

Full Text:

PDF

References


HPA (2012a). English National Point Prevalence Survey on Healthcare- Associated Infections and Antimicrobial Use, 2011: Preliminary Data. London: Health Protection Agency.

Donlan RM. Biofilms and device-associated infections. Emerging infectious diseases. 2001 Mar;7(2):277.

Donlan RM. Biofilms on central venous catheters: is eradication possible?. InBacterial biofilms 2008 (pp. 133-161). Springer Berlin Heidelberg.

Rutala WA, Weber DJ. Disinfection and sterilization: an overview. American journal of infection control. 2013 May 31;41(5):S2-5.

Rajh T, Dimitrijevic NM, Bissonnette M, Koritarov T, Konda V. Titanium dioxide in the service of the biomedical revolution. Chemical reviews. 2014 Aug 29;114(19):10177-216.

Hong H, Shi J, Yang Y, Zhang Y, Engle JW, Nickles RJ, Wang X, Cai W. Cancer-targeted optical imaging with fluorescent zinc oxide nanowires. Nano letters. 2011 Aug 10;11(9):3744-50.

Liu Y. Application of Gadolinium-Doped Zinc Oxide Quantum Dots for Magnetic Resonance and Fluorescence Imaging. InMultifunctional Nanoprobes 2018 (pp. 65-79). Springer, Singapore.

Ansari SA, Husain Q, Qayyum S, Azam A. Designing and surface modification of zinc oxide nanoparticles for biomedical applications. Food and Chemical Toxicology. 2011 Sep 1;49(9):2107-15.

Yuan Q, Hein S, Misra RD. New generation of chitosan-encapsulated ZnO quantum dots loaded with drug: synthesis, characterization and in vitro drug delivery response. Acta Biomaterialia. 2010 Jul 1;6(7):2732-9.

Weir A, Westerhoff P, Fabricius L, Hristovski K, Von Goetz N. Titanium dioxide nanoparticles in food and personal care products. Environmental science & technology. 2012 Feb 8; 46(4):2242-50.

Fan Z, Lu JG. Zinc oxide nanostructures: synthesis and properties. Journal of nanoscience and nanotechnology. 2005 Oct 1;5 (10):1561-73.

Mao, Y., Park, T. J., & Wong, S. S. (2005). Synthesis of classes of ternary metal oxide nanostructures. Chemical Communications, (46), 5721-5735.

Yalcinkaya F, Lubasova D. Quantitative evaluation of antibacterial activities of nanoparticles (ZnO, TiO2, ZnO/TiO2, SnO2, CuO, ZrO2, and AgNO3) incorporated into polyvinyl butyral nanofibers. Polymers for Advanced Technologies. 2017 Jan 1;28(1):137-40.

Jašková V, Hochmannová L, Vytřasová J. TiO2 and ZnO nanoparticles in photocatalytic and hygienic coatings. International journal of photoenergy. 2013 Feb 24;2013.

Talebian N, Doudi M, Mogoei H. Antibacterial activities of sol–gel derived ZnO-multilayered thin films: p-NiO heterojunction layer effect. Journal of Sol-Gel Science and Technology. 2015 Jun 1;74(3):650-60.

Widiarti N, Sae JK, Wahyuni S. Synthesis CuO-ZnO nanocomposite and its application as an antibacterial agent. InIOP Conference Series: Materials Science and Engineering 2017 Feb (Vol. 172, No. 1, p. 012036). IOP Publishing.

Xu JW, Gao ZD, Han K, Liu Y, Song YY. Synthesis of magnetically separable Ag3PO4/TiO2/Fe3O4 heterostructure with enhanced photocatalytic performance under visible light for photoinactivation of bacteria. ACS applied materials & interfaces. 2014 Aug 27;6(17):15122-31.

Edition AS. CLSI document M02-A11. Wayne, PA: Clinical and Laboratory Standards Institute. 2012;32(1):76.

Jesline A, John NP, Narayanan PM, Vani C, Murugan S. Antimicrobial activity of zinc and titanium dioxide nanoparticles against biofilm-producing methicillin-resistant Staphylococcus aureus. Applied Nanoscience. 2015 Feb 1;5(2):157-62.

Joe A, Park SH, Shim KD, Kim DJ, Jhee KH, Lee HW, Heo CH, Kim HM, Jang ES. Antibacterial mechanism of ZnO nanoparticles under dark conditions. Journal of Industrial and Engineering Chemistry. 2017 Jan 25;45:430-9.

Hirota K, Sugimoto M, Kato M, Tsukagoshi K, Tanigawa T, Sugimoto H. Preparation of zinc oxide

ceramics with a sustainable antibacterial activity under dark conditions. Ceramics International. 2010 Mar 31;36(2):497-506.

Kirkinezos IG, Moraes CT. Reactive oxygen species and mitochondrial diseases. InSeminars in cell & developmental biology 2001 Dec 31 (Vol. 12, No. 6, pp. 449-457). Academic Press.

Clifton LA, Skoda MW, Daulton EL, Hughes AV, Le Brun AP, Lakey JH, Holt SA. Asymmetric phospholipid: lipopolysaccharide bilayers; a Gram-negative bacterial outer membrane mimic. Journal of The Royal Society Interface. 2013 Dec 6;10(89):20130810.

Santos RS, Figueiredo C, Azevedo NF, Braeckmans K, De Smedt SC. Nanomaterials and molecular transporters to overcome the bacterial envelope barrier: Towards advanced delivery of antibiotics. Advanced drug delivery reviews. 2017 Dec 14.Egan, A. J. (2018). Bacterial outer membrane constriction. Molecular microbiology.

Bajaj H, Acosta Gutierrez S, Bodrenko I, Malloci G, Scorciapino MA, Winterhalter M, Ceccarelli M. Bacterial outer membrane porins as electrostatic nanosieves: exploring transport rules of small polar molecules. ACS nano. 2017 May 12;11(6):5465-73

Auer GK, Weibel DB. Bacterial cell mechanics. Biochemistry. 2017 Jul 11;56(29):3710-24




Copyright (c) 2018 Journal of Biomedical and Clinical Sciences (JBCS)

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 

Flag Counter           

                     

                                              Copyright © 2016 AMDI Publisher, Universiti Sains Malaysia.
Disclaimer : This website has been updated to the best of our knowledge to be accurate. However, Universiti Sains Malaysia shall not be liable for any loss or damage caused by the usage of any information obtained from this web site.
                                            Best viewed: Mozilla Firefox 4.0 & Google Chrome at 1024 × 768 resolution.